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Figure 4 | BMC Plant Biology

Figure 4

From: Selenite activates the alternative oxidase pathway and alters primary metabolism in Brassica napus roots: evidence of a mitochondrial stress response

Figure 4

The effects of selenite on the ubiquitin-proteasome pathway in root tissue. (a) Proteasome activity in roots of untreated plants and plants treated with 50 μM selenite for 1 and 3 d. Shown are the mean and SE in 5 different plants. Values represent fluorescence of proteasomally-released AMC at the each time interval. Data are the mean of three biological replicates and standard deviation. Lowercase letters represent a significant difference in activity at each time point (p < 0.05). (b) The accumulation of high-molecular weight ubiquitinated proteins in the roots of B. napus from untreated plants and plants treated with 50 μM selenite for 1 and 3 days, and then supplemented in 0.1% DMSO with or without 100 μM MG132 in for 8 hours. 50 μg of protein were separated on an 8% SDS gel, and ubiquitinated proteins were detected using anti-ubiquitin antiserum. The immunoblot is representative of at least three biological experiments, and numbers below each blot represent the mean pixel intensity of all the immunoreactive bands relative to control on day 1. Asterisks indicate a significant difference in band intensity in selenite-treated plants compared to untreated plants (p < 0.05). L = ladder.

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