Feeding preference of S. exigua larvae is determined by terpene content. (A) Dual-choice test for feeding preference comparing trichome-cured wild-type (blue) and OPR3-RNAi leaves (green) were performed as in Figure 2B. The consumed leaf area is shown in percent as the mean +/- SD of 58 experiments. Differences between the means are not significant (Wilcoxon signed rank test: P = 0.895). (B) Dual-choice tests comparing artificial diet to which cis-3-hexenal (n = 44) or a blend of mono- and sesquiterpenes (n = 86) were added in concentrations reflecting the content of wild-type (blue) or OPR3-RNAi trichomes (green). Diet consumption after 20 hrs is shown in percent as the mean +/- SD. Asterisks indicate significant preference (Wilcoxon signed rank test: ***P < 0.001).