S. exigua prefers JA-deficient over wild-type plants for oviposition and feeding. (A) Oviposition preference was analyzed in dual-choice assays as the number of egg deposits on OPR3-RNAi (green bars) as compared to wild-type plants (WT1, UC82B; blue bars). Data are shown for three independent OPR3-RNAi lines individually on the left (lines A15, A52, and P3), and as the mean of the three lines +/- SD on the right (paired t-test: **P = 0.007). (B) Feeding preference was analyzed in dual-choice assays using three independent OPR3-RNAi lines (green) and the jai1 mutant (yellow) with the corresponding wild types (WT1, UC82B; WT2, Castlemart; blue). Each experiment consisted of three mutant and wild-type leaf discs offered to three larvae for feeding. Preference is shown as percent consumed leaf area after four hours. Data represent the mean +/- SD of at least 20 replicates (n = 28, 27, 37, and 20 for J55, J18, A52, and the jai1 mutant). Asterisks indicate significant preference (Wilcoxon signed rank test: ***P < 0.001).