In vivo characterization of MsSN1 overexpressing transgenic plants for resistance to Phoma medicaginis CT1. (A) Number of diseased leaflets related to total number of leaflet in 30-day-old inoculated plants. (B) Percentage of two-month-old inoculated plants with regrowth. (C) Percentage of vigor affected two-month-old inoculated plants. (D) Percentage of highly defoliated two-month-old inoculated plants (highly defoliated > 3 leaflets detached). (E) Representative photo comparing transgenic (S) with wild type (Wt) 30-day-old inoculated plants. (F) Detail of regrowth. (G) Detail of symptomatic regrowth. S: MsSN1-overexpressing plants. Wt: wild type. All values are means ± SEM (n =15-25). N.S: non-significant; *p < 0.05; **p < 0.01; ***p < 0.001, t-test.