Antifungal in vitro activity of MsSN1 overexpressing transgenic plants. (A) Diseased leaflets related to leaflets inoculated with Phoma (#diseased leaflet/#total leaflet). (B) Diseased leaflets related to leaflets inoculated with Colletotrichum (#diseased leaflet/#total leaflet). (C) Damage score: 1. Healthy leaflet, 2. Countable injuries, 3. Uncountable injuries, 4. Chlorosis, 5. completely damaged. (D) Representative Phoma assay. Wt: wild type. S1-S3: MsSN1 transgenic plants. All values are log means ± SEM (n = 10–30). Asterisks indicate a statistically significant difference (Turkey: *p < 0.5; **p < 0.01 ***p < 0.001). Leaflets were considered diseased when they showed symptoms 3, 4 or 5 in the damage score. Disease severity was estimated from scoring 30 to 60 detached leaflets from three individual plants.