Figure 7
Confocal microscope images of wild type C. reinhardtii (cc124) incubated with the GFP-V H H B11 chimeric nanobody. A) Cells detected in the GFP fluorescence channel displaying specific staining of the cell walls. D) Cells incubated with a GFP-VHH B5 (negative control) showing no fluorescence. A and D: GFP fluorescence channel, B and E: chloroplast auto-fluorescence channel; C and F: phase contrast images of cells.