Figure 3

ROS production in rice. (a) Detection of singlet oxygen in leaves, as monitored by increase in SOSG fluorescence emission at 530 nm. Leaf segments were vacuum infiltrated with 200 μM SOSG solution before being illuminated at 2,000 μmol photons m^-2 s^-1. (b) Production of superoxide anion radicals. Histochemical staining with NBT in wild-type (WT), PsbS-KO and PsbS-RNAi leaves incubated under darkness for 2 h (Dark), under moderate light at 200 -mol photons m^-2 s^-1 (LL), or under photoinhibitory illumination at 2,000 μmol photons m^-2 s^-1 (HL). (c) Production of hydrogen peroxide. Histochemical staining with DAB in wild-type (WT), PsbS-KO and PsbS-RNAi leaves under control conditions (Dark), under moderate light at 200 μmol photons m^-2 s^-1 (LL), or after 2 h of photoinhibitory illumination at 2,000 μmol photons m^-2 s^-1 (HL). Experiments were repeated 4-6 times and representative images shown.