Characterization of proanthocyanidin metabolism in pea (Pisum sativum) seeds

BMC Plant Biology

Table 1 PA chemical analyses of `Courier', `Solido', and `LAN3017' pea seeds and seed coats

PA analysis using phloroglucinolysis and RP-HPLC-DAD in mature pea seeds
Peak ID	Compound	`Courier'	`Solido'	`LAN3017'
3	GC-P	29.23 ± 0.73^a	28.99 ± 0.88	1.36 ± 0.02
4	EGC-P	55.38 ± 1.05	51.16 ± 1.27	0.79 ± 0.05
5	GC	9.88 ± 0.29	10.53 ± 0.14	nd^b
6	CT-P isomer	nd	nd	4.98 ± 0.00
7	CT-P	nd	0.22 ± 0.01	21.41 ± 0.01
8	EC-P	0.37 ± 0.01	0.60 ± 0.02	65.37 ± 0.03
9	EGC	5.13 ± 0.04	8.24 ± 0.21	nd
10	CT	nd	nd	0.94 ± 0.00
11	EC	nd	0.27 ± 0.02	5.15 ± 0.02
mDP		6.7 ± 0.2	5.3 ± 0.1	16.4 ± 0.0
Conversion yield^c		83.9 ± 1.6	78.3 ± 4.9	59.1 ± 0.9
Total seed PA^d		416.0 ± 7.7	264.1 ± 14.6	96.7 ± 13.2
Butanol-HCl quantification of PA content from pea seed coats
Total seed coat PA (%)^e		4.57 ± 0.03	4.51 ± 0.09	5.10 ± 0.07

^aMolar % ± SE (n = 2); ^bnd, not detected; ^cYield of PA extract calculated.
^dTotal seed PA content based on characterized PA subunits, expressed as mg/100 g dry weight of whole seeds. GC-P, gallocatechin-(4α → 2)-phloroglucinol; GC, gallocatechin; EGC-P, epigallocatechin-(4β → 2)-phloroglucinol; EGC, epigallocatechin; CT-P, catechin-(4α →2)-phloroglucinol; CT, catechin; EC-P, epicatechin-(4β → 2)-phloroglucinol; EC, epicatechin.
^eTotal seed coat PA content expressed as % = mg/100 mg dry weight of seed coat sample using 80% methanol extraction. Proanthocyanidin extract from `CDC Acer' pea seed coats purified as described by Jin et al. [41] was used as a standard for the butanol-HCl assay. Data are means ± SE (n = 3).

ISSN: 1471-2229