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Table 4 Primer set designed for RT-qPCR

From: Genome-wide annotation of the soybean WRKY family and functional characterization of genes involved in response to Phakopsora pachyrhiziinfection

Target

Orientation

Tm (°C)*

Primer Sequence

PCR product size (pb)

GmWRKY20 transcripts

Forward

60

5'-TTGCAAAGTTCAGAAGTATCTTGTC-3'

264

Reverse

60

5'-GTGACCTGTTGTAGATCCCATC-3'

 

GmWRKY27 transcripts

Forward

61.69

5'-GATTGTGCATTTGCTAATCATGC-3'

105

Reverse

59.93

5'- GCTATAGAAACTTCGCCAGAAC-3'

 

GmWRKY46 transcripts

Reverse

60

5'-CAATGCATCATCAACTTCCG-3'

213

Forward

60

5'-CAAGACCACTTTCACAGCTCAC-3'

 

GmWRKY56 transcripts

Forward

60.06

5'-CACCCATCTGCCTCATCAC-3'

234

Reverse

59.17

5'-GGAGGCCGAGTCTGTACAAT-3'

 

GmWRKY57 transcripts

Forward

60

5'-TCCCTCAACTTCCTCCAATC-3'

170

Reverse

60

5'-GGAAGGGTTCAAAGGCATC-3'

 

GmWRKY106 transcripts

Forward

59.72

5'-GGAAATAAAGTTCCACTAAGGATGAC-3'

174

Reverse

60.57

5' - CCGAGAATGTGTGCTACAACC-3'

 

GmWRKY125 transcripts

Forward

60

5'-TCTCATCTTCCAATAATTTCCCA-3'

135

Reverse

60

5'-CATGATGCCTTGGTGAGCTA-3'

 

GmWRKY139 transcripts

Forward

60.53

5'-CAAATCCTTTTGGTGGGAATC-3'

145

Reverse

59.30

5'-CTATAGAAATTTCGCAAGAACTTAACC-3'

 

Metalloprotease transcripts

Forward

60.5

5'-ATGAATGACGGTTCCCATGTA-3'

114

Reverse

60.17

5'-GGCATTAAGGCAGCTCACTCT-3'

 

FBox transcripts

Forward

60.25

5'-AGATAGGGAAATGTTGCAGGT-3'

93

Reverse

59.84

5'-CTAATGGCAATTGCAGCTCTC-3'

 
  1. *Calculated Tm under PCR conditions.