Target | Orientation | Tm (°C)* | Primer Sequence | PCR product size (pb) |
---|---|---|---|---|
GmWRKY20 transcripts | Forward | 60 | 5'-TTGCAAAGTTCAGAAGTATCTTGTC-3' | 264 |
Reverse | 60 | 5'-GTGACCTGTTGTAGATCCCATC-3' | Â | |
GmWRKY27 transcripts | Forward | 61.69 | 5'-GATTGTGCATTTGCTAATCATGC-3' | 105 |
Reverse | 59.93 | 5'- GCTATAGAAACTTCGCCAGAAC-3' | Â | |
GmWRKY46 transcripts | Reverse | 60 | 5'-CAATGCATCATCAACTTCCG-3' | 213 |
Forward | 60 | 5'-CAAGACCACTTTCACAGCTCAC-3' | Â | |
GmWRKY56 transcripts | Forward | 60.06 | 5'-CACCCATCTGCCTCATCAC-3' | 234 |
Reverse | 59.17 | 5'-GGAGGCCGAGTCTGTACAAT-3' | Â | |
GmWRKY57 transcripts | Forward | 60 | 5'-TCCCTCAACTTCCTCCAATC-3' | 170 |
Reverse | 60 | 5'-GGAAGGGTTCAAAGGCATC-3' | Â | |
GmWRKY106 transcripts | Forward | 59.72 | 5'-GGAAATAAAGTTCCACTAAGGATGAC-3' | 174 |
Reverse | 60.57 | 5' - CCGAGAATGTGTGCTACAACC-3' | Â | |
GmWRKY125 transcripts | Forward | 60 | 5'-TCTCATCTTCCAATAATTTCCCA-3' | 135 |
Reverse | 60 | 5'-CATGATGCCTTGGTGAGCTA-3' | Â | |
GmWRKY139 transcripts | Forward | 60.53 | 5'-CAAATCCTTTTGGTGGGAATC-3' | 145 |
Reverse | 59.30 | 5'-CTATAGAAATTTCGCAAGAACTTAACC-3' | Â | |
Metalloprotease transcripts | Forward | 60.5 | 5'-ATGAATGACGGTTCCCATGTA-3' | 114 |
Reverse | 60.17 | 5'-GGCATTAAGGCAGCTCACTCT-3' | Â | |
FBox transcripts | Forward | 60.25 | 5'-AGATAGGGAAATGTTGCAGGT-3' | 93 |
Reverse | 59.84 | 5'-CTAATGGCAATTGCAGCTCTC-3' | Â |