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Figure 7 | BMC Plant Biology

Figure 7

From: Genome-wide annotation of the soybean WRKY family and functional characterization of genes involved in response to Phakopsora pachyrhiziinfection

Figure 7

T-DNA region of binary vectors used for Gm WRKY27 overexpression or Gm WRKY silence in soybean. (A) Overexpression construct - pH7WG2D.1-GmWRKY27. The full-length ORF of GmWRKY27 was cloned in the vector. (B) RNAi suppression construct - pH7GWIWG2(II).0-GmWRKY. Inverted repeats of a 176-bp WRKY fragment was cloned into the vector. RB - T-DNA right border, LB - left border, hpt - hygromycin phosphotransferase gene, P35S - Cauliflower mosaic virus (CaMV) 35S promoter, T35S - CaMV 35S terminator, EgfpER - enhanced green fluorescent protein, ProlD - root loci D promoter, WRKY - soybean 176 pb WRKY fragment, attB1 and attB2 - LR reaction site.

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