Figure 5From: Genome-wide annotation of the soybean WRKY family and functional characterization of genes involved in response to Phakopsora pachyrhiziinfectionP. pachyrhizi development on the detached leaves at 12 days after inoculation. Three detached leaves of each one transgenic line and two wild-type plants were inoculated with 105/mL spore suspension and incubated at 20°C. (A) Two infection parameters were evaluated: number of lesions and number of pustules. *Means are significantly different in leaves of wild type (wt) and transgenic soybean line P3-2. (Student's t-test, p < 0.05). (B) Low number of tan-colored lesions and pustules under stereomicroscope in a leaf of wild-type (wt) plant. (C) High number of tan-colored lesions and pustules under stereomicroscope in a leaf of transgenic soybean line P3-2 with suppression of the four WRKYs.Back to article page