Table 3 Effect of different explant types and selection methods on transformation frequencies in crambe
Explant type | Medium | Selection | No. of explants | No. of explants with regenerating shoots | No. of explants with GUS + regenerating shoots | Transformation frequency |
|---|---|---|---|---|---|---|
Cotyledonary node | A | Shifting | 111 | 17 | 11 | 10% |
Consistent | 116 | 1 | 1 | 1% | ||
B | Shifting | 114 | 16 | 4 | 4% | |
Consistent | 110 | 0 | 0 | 0% | ||
Cotyledon | A | Shifting | 106 | 4 | 4 | 4% |
Consistent | 104 | 1 | 1 | 1% | ||
B | Shifting | 110 | 0 | 0 | 0% | |
Consistent | 98 | 0 | 0 | 0% |