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Figure 2 | BMC Plant Biology

Figure 2

From: Impact of swapping soils on the endophytic bacterial communities of pre-domesticated, ancient and modern maize

Figure 2

16S rDNA TRFLP profiles of the bacterial endophytic communities inhabiting young Zea plants based on a culture-independent approach. Shown are fluorescently labelled (6FAM) 799f fragments of bacterial DNA from: (A) shoot tissues and (B) root tissues growing in different soils. Each peak is the fluorescence intensity average of six TRFLP amplifications from three pools of five plants, a semi-quantitative indicator of microbial titre. Mixteco plants grown in sand are the average of four TRFLP amplifications from two pools of 5 plants. 16S rDNA amplicons were generated using primers 799f/1492rh and then were restricted using DdeI. Small fragments and those corresponding to 16S chloroplast rDNA or 18S rDNA were removed from the display. Max550 labelled fragments are not shown.

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BMC Plant Biology

ISSN: 1471-2229

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