Figure 8

Phenotyping the resistance to Bean pod mottle virus (BPMV) in P. vulgaris RILs and genetic mapping of the R-BPMV gene on the integrated linkage map. (A) Phenotyping the resistance to BPMV was achieved in BPMV-GFP inoculated plants by visual inspection of inoculated leaves under UV light at 7 days post-inoculation (dpi). Representative inoculated leaves are shown for the two parental lines (BAT93 and JaloEEP558) and a sample of 10 RILs. (B) Semi-quantitative RT-PCR of BPMV RNA1 and RNA2 in systemic leaves of 10 RILs inoculated with the empty BPMV vector (BPMV-0). Ubiquitin (PvUBI) was used as an internal control. Total RNA was extracted at 28 dpi from a pool of three trifoliate leaves from three different plants of each RIL. (C) Location of R-BPMV on LG B2 based on the 77 BAT93 X JaloEEP558 RILs population [29],[44],[45]. Marker names are indicated on the right of the linkage group. Map distances are indicated between molecular markers and expressed in Kosambi centiMorgans.