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Figure 7 | BMC Plant Biology

Figure 7

From: The "one-step" Bean pod mottle virus (BPMV)-derived vector is a functional genomics tool for efficient overexpression of heterologous protein, virus-induced gene silencing and genetic mapping of BPMV R-gene in common bean (Phaseolus vulgaris L.)

Figure 7

Virus-induced gene silencing of PvPDS in P. vulgaris cv. JaloEEP558 using the BPMV-PvPDS-391 bp vector. (A) Plants inoculated with mock buffer (first left panel), BPMV-0 (middle panel), and BPMV-PvPDS-391 bp were photographed under natural light at 7 weeks post-inoculation (wpi). Representative trifoliate leaves of the corresponding plants are represented in the lower panel. (B) Semi-quantitative RT-PCR of BPMV RNA1 (upper panel) and RNA2 (two middle panels) in systemic leaves of plants inoculated with mock buffer, BPMV-PvPDS-391 bp and BPMV-0. Ubiquitin (PvUBI) was used as an internal control. Total RNA was extracted at 7 wpi from a pool of three fourth trifoliate leaf of three different plants of JaloEEP558.

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