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Figure 5 | BMC Plant Biology

Figure 5

From: The "one-step" Bean pod mottle virus (BPMV)-derived vector is a functional genomics tool for efficient overexpression of heterologous protein, virus-induced gene silencing and genetic mapping of BPMV R-gene in common bean (Phaseolus vulgaris L.)

Figure 5

Silencing of PDS in P. vulgaris cv. Black Valentine using the VIGS vector BPMV-GmPDS-327 bp. (A-B) Plants inoculated with mock buffer (left panel), BPMV-0 (middle panel), and BPMV-GmPDS-327 bp (right panel) were photographed under natural light at 28 days post-inoculation (dpi). (B) Trifoliate systemic leaves. (C) Semi-quantitative RT-PCR of PvPDS, BPMV RNA1 and RNA2 in systemic leaves of plants inoculated with mock, BPMV-0, and BPMV-GmPDS-327 bp. Ubiquitin (PvUBI) was used as an internal control. Total RNA was extracted at 21 dpi from the third trifoliate leaf of three different plants of Black Valentine.

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BMC Plant Biology

ISSN: 1471-2229

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