Figure 4From: The "one-step" Bean pod mottle virus (BPMV)-derived vector is a functional genomics tool for efficient overexpression of heterologous protein, virus-induced gene silencing and genetic mapping of BPMV R-gene in common bean (Phaseolus vulgaris L.)Bean pod mottle virus (BPMV)-induced expression of the green fluorescent protein ( GFP ) gene in leaves of P. vulgaris genotypes of interest. (A) BPMV-GFP inoculated plants were photographed under UV light, at 7 days post-inoculation (dpi) for inoculated leaves, and at 21 dpi and 28 dpi for systemic leaves. (B-C) Semi-quantitative RT-PCR of BPMV RNA1 and RNA2 in inoculated leaves (B) and systemic leaves (C) of control plants (mock treatment) and plants inoculated with BPMV-GFP. Ubiquitin (PvUBI) was used as an internal control. Total RNA was extracted at 9 dpi from the inoculated leaves and at 30 dpi from the fourth trifoliate and third trifoliate leaves of three different plants of Black Valentine and JaloEEP558, respectively.Back to article page