Figure 4

Induction of SPL7 targets following ER stress. Arabidopsis wild-type (WT) and spl7-2 mutant seedlings grown on ½ MS supplemented with CuSO₄ 0.5 μM for 5 days were transferred to liquid ½ MS (control; cont) or to liquid ½ MS supplemented with tunicamycin (5 μg/mL; tuni) or DTT (2 mM; DTT) for 3 hours. Total RNA was isolated and relative transcript levels of selected SPL7 targets monitored by qPCR. Error bars indicate standard deviation (n ≥ 3 independent biological samples), asterisks statistically significant difference to control conditions in Student's t-test (* p < 0.05; ** p < 0.01).