Influence of ClpT1 in the ATPase activity of ClpC2 and ClpD. Release of inorganic phosphate was monitored spectrophotometrically by the Malachite green method. The% activity of each chaperone in the presence of varying amounts of ClpT1 was calculated as [specific activity (nmoles inorganic phosphate/minxμg protein) in the presence of ClpT1 x 100/specific activity in the absence of ClpT1]. The% activity is plotted as a function of the log[ClpT1]/[Hsp100] molar ratio. The additional axes on the right show the scale of specific activity of ClpC2 and ClpD. The experiments were performed in triplicate.