Interaction of ClpT1 with chloroplastic Hsp100 chaperones from Arabidopsis . ClpT1 was incubated for 10 min with the chaperones ClpC2 (B) and ClpD (C) in the presence of 5 mM ATP and subjected to ultrafiltration for 30 s. The experimental setup is shown in (A). The permeate (P) and the retentate (R) were collected and analyzed by SDS-PAGE. Gels were stained with Coomassie Brilliant Blue. GFP was used as a control. The amount of each protein was quantified by gel densitometry using the software GelPro and plotted as a bar chart (standard deviation bars are indicated). Experiments were performed in triplicate; the pictures show a representative result. Bands were cropped from the complete gel image for the sake of clarity. This image is provided in the Additional file 2: Figure S2.