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Table 1 Transcripts that were both pathogen-responsive and differentially regulated (≥1.5 fold) in the uzu derivative of barley cultivar Akashinriki-uzu, as compared to parent type barley line

From: Plant disease resistance is augmented in uzu barley lines modified in the brassinosteroid receptor BRI1

Sequence Code# Microarray data comparisons Gene Ontology* BEST BLASTX±
Fold induction (uzu vs Aka)¥ P-value Fold induction (uzu + fungus vs uzu + no fungus)¥ P-value Gene/protein ID Annotation Evalue Organism
Transcripts that were both pathogen up-regulated and up-regulated in uzu versus the parent
Contig5883_s 2.35282 9.89E-04 3.47814 4.86E-06 GO:0000162 AAM19104.1 Phosphoribosylanthranilate transferase 4.00E-35 Oryza sativa
Contig10483 1.77649 4.93E-04 2.03916 5.33E-05 GO:0016491 NP_177122.2 Acid phosphatase 7.00E-22 Arabidopsis thaliana
Contig12753 6.85586 3.48E-04 8.65154 1.60E-04 GO:0000166 BAB93292.1 Putative ABC transporter 4.00E-75 Oryza sativa
Contig10167 4.05623 1.60E-05 4.42652 1.38E-05 GO:0003700 BAB93351.1 Hypothetical protein B1131B07.13 6.00E-43 Oryza sativa
Contig11212 15.30721 8.05E-06 8.85902 2.01E-04 GO:0003824 AAL58118.1 Putative flavanone 3-hydroxylase 1.00E-72 Oryza sativa
HV_CEb0014M10r2 2.53882 4.62E-04 3.69271 1.20E-05 GO:0003824 AAF89745.3 Phosphatidic acid phosphatase beta 2.00E-15 Vigna unguiculata
Contig1179 3.15308 7.72E-04 3.69557 6.89E-04 GO:0005634 P05621 Histone H2B.2 5.00E-44 Triticum aestivum
Contig13350 8.37292 2.88E-08 38.39412 8.05E-12 GO:0005975 AAD28734.1 β-1,3-glucanase precursor 7.00E-65 Triticum aestivum
HV_CEb0022J21r2 4.86722 4.44E-04 10.55195 5.40E-05 GO:0006857 AAG46153.1 Putative peptide transporter 2.00E-32 Oryza sativa
HVSMEm0003C15r2_s 4.03305 1.11E-05 9.02364 1.73E-09 GO:0006952 A31800 Glucan endo-1,3-beta-D-glucosidase 2.00E-48 Hordeum vulgare
Contig10115 2.49729 9.01E-04 4.69131 1.10E-06 GO:0009058 NP_199633.1 Indole-3-glycerol phosphate synthase 1.00E-62 Arabidopsis thaliana
Contig18088 2.24148 9.93E-05 2.74884 9.52E-06 GO:0009536 S39045 Probable finger protein WZF1 2.00E-35 Triticum aestivum
Contig5542 2.61205 8.75E-04 2.91793 3.14E-04 GO:0009536 AAG42689.1 Putative tryptophan synthase α 1.00E-65 Zea mays
Contig21646 6.15205 6.18E-06 5.32979 4.06E-05 GO:0015297 BAC20746.1 Putative integral membrane protein 1.00E-26 Oryza sativa
Contig7898 2.74868 2.24E-04 2.68024 9.67E-04 GO:0016020 BAB64118.1 Hypothetical protein P0039A07.26 1.00E-62 Oryza sativa
Contig12237 3.62694 2.18E-08 7.23322 7.95E-12 GO:0016023 Q9M5X7 Nonspecific lipid-transfer protein precursor 2.00E-09 Malus domestica
Contig1636 2.83333 1.53E-05 3.82892 1.95E-07 GO:0016023 Q02126 Glucan endo-1,3-beta-glucosidase GIII e-164 Hordeum vulgare
Contig1637 3.92582 1.23E-04 10.52758 2.30E-08 GO:0016023 D38664 Glucan endo-1,3-beta-D-glucosidase e-162 Hordeum vulgare
Contig1637_s 3.72453 1.04E-04 6.92245 1.89E-07 GO:0016023 D38664 Glucan endo-1,3-beta-D-glucosidase e-162 Hordeum vulgare
Contig1639 5.68725 2.62E-05 6.68401 8.82E-06 GO:0016023 S35156 β-glucanase e-106 Hordeum vulgare
Contig2212_s 4.33587 3.48E-05 7.27081 3.89E-05 GO:0016023 P35793 Pathogenesis-related protein PRB1-3 8.00E-88 Hordeum vulgare
Contig26496 2.99884 5.20E-04 17.90724 4.22E-16 GO:0016023   No hit   
Contig2790_s 2.12349 1.71E-05 2.17092 5.04E-05 GO:0016023 AAK55325.1 Thaumatin-like protein TLP7 5.00E-74 Hordeum vulgare
Contig2990 2.32856 7.50E-04 4.22865 2.14E-06 GO:0016023 S48847 Chitinase e-134 Hordeum vulgare
Contig3667_s 2.61309 8.97E-04 3.87741 9.78E-06 GO:0016023 T06179 MYB-related protein e-164 Hordeum vulgare
Contig3947_s 2.40807 4.30E-04 3.94464 4.87E-07 GO:0016023 AAK55323.1 Thaumatin-like protein TLP4 5.00E-71 Hordeum vulgare
Contig4174 2.60929 2.83E-04 4.31725 6.89E-07 GO:0016023 S39979 Chitinase 1.00E-66 Oryza sativa
Contig6007 3.03741 2.95E-04 4.3984 1.99E-05 GO:0016023 BAB63883.1 Putative bromelain-like thiol protaease 7.00E-48 Oryza sativa
Contig2210/ Contig4054_s 2.816 3.17E-04 10.06151 8.75E-11 GO:0006952 Q05968 Pathogenesis-related protein 1 2E-80 Hordeum vulgare
Contig2550_x 2.11143 9.56E-04 3.1568 1.71E-05 GO:0016998 T06169 Pathogenesis-related protein 4 8.00E-77 Hordeum vulgare
Contig6576 2.21974 8.26E-04 4.15007 3.54E-05 GO:0016998 T06169 Pathogenesis-related protein 4 1.00E-50 Hordeum vulgare
Contig6576_s 2.92619 4.27E-04 5.06554 1.51E-06 GO:0016998 T06169 Pathogenesis-related protein 4 1.00E-50 Hordeum vulgare
Contig18116 3.68173 2.25E-04 6.95245 6.21E-07 GO:0043169 NP_181494.1 Glycosyl hydrolase family 17 1.00E-30 Arabidopsis thaliana
Contig2170/ Contig5974_s 18.26831 1.03E-11 11.48664 5.37-E06 GO:0016021 Q01482 Pathogen-induced protein WIR1A 3.00E-08 Triticum aestivum
HV_CEb0017C08r2 12.97883 4.83E-07 17.09899 3.83E-07 GO:0046274 AAK37826.1 Laccase 1.00E-19 Pinus taeda
Contig7354 2.06008 1.15E-04 2.04682 2.71E-04 GO:0050660 NP_567283.1 Protein id: At4g05020.1 4.00E-89 Arabidopsis thaliana
Contig13114 3.63475 9.89E-04 5.33272 8.45E-05 No hit AAG21913.1 Putative cyanase 7.00E-08 Oryza sativa
Contig14625 3.09771 4.86E-04 3.05281 7.41E-04 No hit   No hit   
EBem10_SQ002_I10_s 3.3918 7.91E-08 3.93247 6.25E-08 No hit AAK55326.1 Thaumatin-like protein TLP8 8.00E-04 Hordeum vulgare
EBro08_SQ012_C01 3.10739 4.52E-05 3.48265 2.89E-04 No hit   No hit   
HO04B16S 1.72257 6.40E-05 1.58429 0.00012 GO:0008152 BAB16426.1 Elicitor inducible gene product EIG-I24 2E-20 Nicotiana tabacum
Contig12169 1.9972 0.000228 1.62783 0.000548 GO:0016020 AAM93464.1 Unknown protein 9E-89 Oryza sativa
Contig6004 1.69354 1.41E-04 2.61521 5.12E-04 No hit NP_564147.1 Unknown protein 2.00E-10 Arabidopsis thaliana
Contig2500 1.52109 0.000730 1.54984 0.000443 No hit BAB07974.1 Unknown protein 1E-42 Oryza sativa
Transcripts that were both pathogen down-regulated and down-regulated in uzu versus the parent
Contig7790 −4.25752 5.86E-05 −4.22732 8.49E-06 GO:0003676 NP_564325.1 Protein id: At1g29250.1 9.00E-44 Arabidopsis thaliana
Contig21684 −9.02614 7.66E-08 −2.09451 1.51E-04 GO:0016762 AAM62971.1 Putative xyloglucan endotransglycosylase 4.00E-34 Arabidopsis thaliana
Contig12965 −2.31955 9.43E-04 −7.42733 3.19E-06 GO:0005525 NP_192854.1 Translation initiation factor IF-2 1.00E-71 Arabidopsis thaliana
Contig4431_s −2.82605 2.50E-10 −2.81901 9.86E-05 No hit AAK44146.1 Unknown protein 1.00E-08 Arabidopsis thaliana
Contig17319 −1.84164 9.67E-04 −2.18675 1.31E-05 GO:0003723 BAC05662.1 Unknown protein 2.00E-40 Oryza sativa
Contig16536 −1.77974 6.09E-04 −1.91262 2.94E-04 GO:0003735 NP_189267.1 Unknown protein 1.00E-16 Arabidopsis thaliana
  1. #Sequences of Affymetrix probes used in the microarray analysis were obtained using probe ID from http://www.plexdb.org.
  2. ±The homologs of barley transcript sequences from the barley database (http://www.plexdb.org) were determined by BLASTx analysis against the non-redundant protein database using the NCBI blast resource (http://www.ncbi.nlm.nih.gov).
  3. ¥Fold induction indicated up-regulation of gene in uzu compare to Akashinriki or uzu + fungus compare to uzu + no fungus.
  4. *Gene ontology was analysed using the web-based tool agriGo http://bioinfo.cau.edu.cn/agriGO/.