Transcript levels of BRI1 downstream and brassinosteroid metabolic genes in epibrassinolide (epiBL) treated seedlings of barley cultivars Akashinriki, Bowman and their uzu derivatives (Aka-uzu and Bow-uzu), as determined by quantitative RT-PCR analysis. Germinating seedlings were treated with Hoaglands solution containing 5 μm of the brassinazole for 4 days and then with Hoaglands solution plus or minus 0.2 μm epiBL. Gene expression was quantified in samples harvested either 12 or 24 h post-treatment. Gene represented are (A) PHYB activation tagged suppressor 1 protein, BAS1 (Contig3160), (B) XET (Contig5258) and (C) ROT3 (Contig12042). Bars indicate SEM (LSD0.05 A = 0.0021, B = 0.016, C = 0.0021).