Skip to main content
Figure 6 | BMC Plant Biology

Figure 6

From: Early markers are present in both embryogenesis pathways from microspores and immature zygotic embryos in cork oak, Quercus suberL

Figure 6

Immunolocalization of auxin (IAA) at early in vitro embryogenesis of microspore and immature zygotic embryos. A-F: Microspore embryogenesis. G-L: Somatic embryogenesis of immature zygotic embryos. A, B, C, G, H, I: Resin sections stained by toluidine blue. D, E, F, J, K, L: Confocal images of IAA immunofluorescence signal (green); in some images, DAPI staining of nuclei (blue) is also shown merged with the green IAA signal (F, K, L). A, D: Microspores (mic). B, E, H, K: Early multicellular embryos (emb). C, F, I, L: Dense cells (arrows) from which early embryos originate at the surface of embryogenic masses (ms). Bars A, D: 50 μm; B, C, E-L: 100 μm.

Back to article page