Figure 6From: Early markers are present in both embryogenesis pathways from microspores and immature zygotic embryos in cork oak, Quercus suberLImmunolocalization of auxin (IAA) at early in vitro embryogenesis of microspore and immature zygotic embryos. A-F: Microspore embryogenesis. G-L: Somatic embryogenesis of immature zygotic embryos. A, B, C, G, H, I: Resin sections stained by toluidine blue. D, E, F, J, K, L: Confocal images of IAA immunofluorescence signal (green); in some images, DAPI staining of nuclei (blue) is also shown merged with the green IAA signal (F, K, L). A, D: Microspores (mic). B, E, H, K: Early multicellular embryos (emb). C, F, I, L: Dense cells (arrows) from which early embryos originate at the surface of embryogenic masses (ms). Bars A, D: 50 μm; B, C, E-L: 100 μm.Back to article page