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Figure 4 | BMC Plant Biology

Figure 4

From: Early markers are present in both embryogenesis pathways from microspores and immature zygotic embryos in cork oak, Quercus suberL

Figure 4

Distribution patterns of non-esterified (JIM5) and highly-esterified (JIM7) pectins during in vitro embryogenesis of microspore and zygotic embryo origins. A, B: Representative immuno dot-blot assays of JIM5 (A) and JIM7 (B) at different developmental stages of microspore embryogenesis. Ponceau Red staining for total proteins and immuno-dot-blot of the same strip is shown for each antibody. C-F: Histograms representing the mean values of dot color intensities in arbitrary units of JIM5 (C, E) and JIM7 (D, F) during microspore embryogenesis (C, D) and somatic embryogenesis of immature zygotic embryos (E, F). Bars on columns indicate standard errors of the means. Different letters on columns indicate significant differences according to ANOVA and Tukey’s test at P ≤ 0.05.

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