Table 1 Sequences of oligonucleotide primers used in this study
Name | Primer sequence | Description |
|---|---|---|
CVY1-F1 | 5’TGCGATGGAGACTGTTTCTCGTGT3’ | For RT-PCR |
CVY1-R1 | 5’ATCAGAGTTTAACCTCGTGGCGGT3’ | For RT-PCR |
TDNA-LB | 5’CCGTCTCACTGGTGAAAAGAA3’ | For TDNA insertion |
CRV1-F2 | 5’ATCATCCCGGGTATCTTCTCCGAATATAGACT3’ | For complementation test (SmaI site italicized) |
CVY1-R2 | 5’CAATTGCCCGGGATATATAATTTAAGCTTCTTTGT3’ | For complementation test (SmaI site italicized) |
Act2-F | 5’GCGGATCCATGGCTGAGGCTGATGATATTCAACC3’ | For RT-PCR |
Act2-R | 5’CGTCTAGACCATGGAACATTTTCTGTGAACGATTCC3’ | For RT-PCR |