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Figure 1 | BMC Plant Biology

Figure 1

From: The Arabidopsis CURVY1 (CVY1) gene encoding a novel receptor-like protein kinase regulates cell morphogenesis, flowering time and seed production

Figure 1

Physical map of CVY1 gene knockout and phenotypic characterization of cvy1 mutant. (A) The CVY1 gene with the positions of the exon (numbered black rectangle) of the gene represented. The 5’ and 3’ untranslated regions are depicted in white rectangles. The location of the Salk T-DNA insertion is shown using an inverted black triangle. The names and locations of primers used for RT-PCR analysis are also indicated. Bar = 0. 5 kb. (B) The T-DNA insertion causes a knockout expression of the gene. The quality of the RNA and the loading control was assayed by monitoring ACTIN gene expression. (C and D) SEM images of upper developing leaves, showing a mature trichome with three branches in wild type (C) and strong distorted trichome in cvy1 (D) plants. (E and F) Confocal images of pavement cell shape pattern of 12 days old WT (E) and cvy1 (F) using lipophilic dye, FM464. Bars = 50 μm (C, D).

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