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Table 1 Electrical Penetration Graph (EPG) parameters considered and their relation to Myzus persicae feeding activity on Arabidopsis thaliana Ws and mutant 3790

From: Constitutive overexpression of the pollen specific gene SKS13 in leaves reduces aphid performance on Arabidopsis thaliana

Related tissue EPG parameter Wild type (Ws) n1 = 18 Mutant 3790 n = 15 Pvalue2
Epidermal Time to first probe (min) 2.5 ± 0.7 2 ± 0.4 0.870
Prephloem Number of probes before first phloem contact 10.5 ± 2.6 13.2 ± 4.6 0.969
  Time from first probe to first phloem contact (min) 61.6 ± 12.4 113.6 ± 15.5 0.024
Phloem Total time of phloem salivation (min) 10.8 ± 1.5 8.1 ± 1.6 0.025
  Number of phloem salivation events 14.3 ± 1.8 10.1 ± 1.8 0.240
  Average duration of phloem salivation (min) 0.8 ± 0.1 0.7 ± 0.1 0.462
  Total time of phloem ingestion (min) 97.5 ± 10.4 33.3 ± 8.2 0.001
  Number of phloem ingestion events 13.3 ± 1.6 8.7 ± 1.7 0.110
  Average duration of phloem ingestion (min) 7.8 ± 0.8 4.3 ± 1.0 0.003
  Total time of sustained (>10 min) phloem ingestion 64.2 ± 9.4 20.4 ± 2.5 0.002
  Number of sustained (>10 min) phloem ingestion 3.9 ± 0.6 1.2 ± 0.3 0.001
  Average duration of sustained (>10 min) phloem ingestion 17.4 ± 2.5 12.7 ± 1.7 0.032
All tissues Total time of non-probing (min) 106.4 ± 16.9 148.3 ± 11.3 0.023
  Total time of pathway phase (min) 247.5 ± 11.5 261.4 ± 13.3 0.278
  Number of aphids with sustained (>10 min) phloem ingestion 18.0 (100%) 11.0 (73%) 0.030
Xylem Total time of G 15.2 ± 5.8 16.2 ± 4.7 0.912
  Number of G 0.7 ± 0.3 0.3 ± 0.2 0.195
  1. Values are means ± SE of EPG parameters during 8 h monitoring. 1EPG replicates; 2Mann Whitney U (duration) or Fisher exact (number) test P values.