Location of the activation tag and expression analysis. (a) Genomic region of mutant 3790 showing the T-DNA insert containing the CaMV35S enhancer. The T-DNA is located in the 3’-UTR (black triangle) of BRL3. The exact distance between the adjacent genes SKS11 and SKS13 with their promoters and the enhancer is unknown. Diagram is not drawn to scale. (b) Quantitative RT-PCR expression analysis of BRL3, SKS11 and SKS13 in rosette leaves of Ws and mutant 3790. Values are the means ± SD (n = 3). The star indicates a significant difference between bars within a pair (Independent-samples t-test, P < 0.05).