Metabolite profiling of whole leaf extract from SnTox3 infiltrated wheat. LC-MS analysis of semi-polar metabolites in leaves infiltrated with SnTox3 or controls at various time points post infiltration. (A) Principal component analysis (PCA) of data showing variation amongst samples and replicates. The SnTox3-infiltrated wheat samples at 48–102 hpi are circled to highlight the separation relative to controls, which indicates a difference in metabolite content at these latter time points. This difference is best described by principal component 2 (PC2). (B) Metabolite loadings of PC2 illustrating the ten metabolites with the highest contribution to the differences between control and SnTox3 infiltrated wheat at 48–102 hpi. All ten metabolite are unknown compounds present at higher levels in SnTox3-infiltrated wheat.