Metabolite profiling of whole leaf extract from SnTox3 infiltrated wheat. GC-MS analysis of polar metabolites in wheat leaves infiltrated with SnTox3 or controls at various time points post infiltration. (A) Principal component analysis (PCA) of data showing variation amongst the metabolite content of samples and replicates. The samples containing SnTox3-infiltrated wheat extracts at 48 and 72 hpi are circled to highlight the clear differentiation in metabolite content compared to controls at these time points. This difference is best described by principal component 1 (PC1). (B) Metabolite loadings of PC1 illustrating ten metabolites with the highest contribution to the differences between control and SnTox3 infiltrated wheat at 48 and 72 hpi. All ten metabolites were present at a higher abundance in SnTox3-infiltrated wheat at these time points.