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Figure 6 | BMC Plant Biology

Figure 6

From: TMV-Cg Coat Protein stabilizes DELLA proteins and in turn negatively modulates salicylic acid-mediated defense pathway during Arabidopsis thalianaviral infection

Figure 6

DELLA proteins are stabilized during TMV-Cg infection and reduce plant growth and delay the timing of floral transition. A) Accumulation of YFP-GAI in the nucleus observed using fluorescence microscope. TMV-Cg infected N. benthamiana leaves and mock-inoculated N. benthamiana leaves were agroinfiltrated with a construction carrying YFP-GAI at 6 dpi. At 72 h post-agroinfiltration leaves were sprayed with 100uMGA3 (GA+) or water (GA-) and subsequently observed an hour later. This experiment was repeated twice with similar results. B) The bar graph shows the levels of accumulation of YFP present in the nuclei of N. benthamiana cells. Quantitative analysis was performed as is explained in Methods. C) The third true leave of non-transgenic Col-0 (grown to stage 1.08) plants were inoculated with TMV-Cg virus. The floral transition and plant growth (plant height at six weeks) were determined in TMV-Cg infected plants as compared to mock-inoculated plants. D) Representative 6-week-old mock-inoculated non- transgenic Col-0 plants and infected non-transgenic Col-0 plants. B) and C) Different letters above the bars represent the significant differences among samples, p-values ≤ 0.05.

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