Figure 5
CgCP expression suppresses a set of defense genes regulated by DELLA proteins. A) Relative mRNA levels determined by RT-qPCR of SA-responsive genes (WRKY70, AOX1A and RDR1) and B) DELLA related defense genes (MPK3, WRKY33, WRKY25 and CBP60g) in gai-1 and quadruple-DELLA mutants compared to WT-Ler plants. The expression level in WT-Ler plants was arbitrarily set to one (Log2(1) = 0). C) Relative mRNA levels of DELLA related defense genes determined by RT-qPCR in induced CP#72 plants (MOF treated) compared to water-treated CP#72(−)control plants over a 72 h time course. The expression level in CP#72(−) plants was arbitrarily set to one (Log2(1) = 0). D) Expression of CBP60g, MPK3, WRKY25 and WRKY33 in CP#71 transgenic line. Relative mRNA levels of individual genes in CP#/71 plants were calculated in comparison to WT-Col-0 plants. Expression level in WT-Col-0 plants was arbitrarily set to one (Log2(1) = 0). A), B), C) and D) The means ± SE of four biological replicates are shown. Asterisks indicate statistically significant differences (* = P values < 0.05).