Figure 4
CgCP expression increases the expression of DELLA targets and stabilizes DELLA accumulation A). Relative mRNA levels of DELLA target genes UBC17, bHLH137, IQD22, ATGID1b, CSD1 and CSD2 were determined by RT-qPCR in induced CP#72 plants (72 h after MOF treatment) compared to water-treated CP#72(−) plants. The expression level in CP#72(−) plants was arbitrarily set to one (Log2(1) = 0). The means ± SE of four biological replicates are shown. Asterisks indicate statistically significant differences (* = P values < 0.05). B) GFP fluorescence observed in induced (MOF) and water- treated (−) CP#72/RGAp::GFP-RGA primary seedling roots. After 3 days of induction (MOF treatment), seedlings were subsequently treated with 10 μMGA3 (+GA) or water (−GA) for 1 h. The experiment was repeated twice with similar results. C) The bar graph shows the levels of accumulation of GFP present in the nuclei of Arabidopsis cells. Quantitative analysis was performed as is explained in Methods. Different letters above the bars represent the significant differences among samples, p-values ≤ 0.05.