Figure 2

CgCP expression downregulates transcriptional levels of SA-responsive genes, but it does not alter endogenous SA or JA levels. A) Relative mRNA levels of AOX1A, RDR1 and WRKY70 genes after CgCP induction in CP#72(MOF) plants (MOF treated) over a 72 h time course. Non-induced CP#72(−) (water treated) were used as reference controls and the expression level was arbitrarily set to one (Log₂ (1) = 0). B) Relative mRNA levels in constitutively expressing CP #71 line compared to WT Col-0 plants at 1.08 developmental stage. The expression level in WT line was arbitrarily set to one (Log₂(1) = 0). C) Relative mRNA levels in CP#72 line after SA treatment. CP#72(−) (water treated) or MOF-treated CP#72 (MOF) plants at 48 h post induction were sprayed either with 0.5 mM SA (+SA) or water (−). The expression levels were referenced to non-induced CP#72(−) plants (water treated) and arbitrarily set to one (Log₂(1) = 0). A), B) and C) Relative transcript levels were determined by RT-qPCR. Data correspond to the mean ± standard error of four biological replicates. Asterisks indicate statistically significant differences (* = P values < 0.05). D) JA and SA accumulation level determined by HPLC in induced CP#72 and non-transgenic Col-0 plants at 0, 24, 48 and 72 h post induction with MOF. Data at 0 h correspond to untreated plants. The means of four biological replicates ± SE are shown.