Changes in protoplast viability and mitochondrial membrane integrity of cassava in the presence of 1 M H
. (a) Viability of cassava mesophyll protoplasts after H2O2 treatment was estimated by fluorescein diacetate (FDA) staining. Data is represented as the mean value of six replicates ± SD (more than 300 cells were counted for each experiment per genotype). **Significant at 1% level from WT by t-test. (b) Mitochondrial membrane integrity under 1 M H2O2 stress. Cassava protoplasts were stained with the rhodamine 123 (Rh123) and the fluorescent signal was observed under a confocal microscope. Scale bar = 5 μm. WT, wild-type control; SA1, SA2 and SA4, independent transgenic plant lines.