High levels of gene flow and genetic diversity in Irish populations of Salix capreaL. inferred from chloroplast and nuclear SSR markers

Table 3 Diversity indicators obtained from the nuclear SSR markers

Locus	N	Pred	Size	N _A	N _E	I	H _O	H _E	HW	F _IS	F _IT	F _ST	G _ST	D _est
SB24	362	109-245	124-182	16	2.8	1.57	0.62	0.64	NS	−0.09^NS	0.03	0.11**	0.05**	0.09**
SB38	364	105-161	106-156	15	9.0	2.34	0.86	0.89	NS	−0.09^NS	0.03	0.11**	0.06**	0.33**
SB85	362	81-87	79-85	3	1.1	0.11	0.04	0.04	NS	−0.12^NS	−0.02	0.09*	0.04*	0.002*
SB93	336	159-185	150-168	7	2.1	0.92	0.07	0.53	S	0.85^NS	0.88	0.23*	0.12*	0.13*
SB194	362	105-152	108-130	11	5.9	1.95	0.75	0.83	S	−0.04^NS	0.09	0.13**	0.07**	0.27**
SB199	212	102-140	98-126	8	2.4	1.20	0.10	0.58	S	0.71^NS	0.79	0.28*	/	/
Mean¹	333			10	3.9	1.35	0.41	0.58		0.08^NS	0.30	0.16**	0.07**	0.10**
SE	24.6			2	1.2	0.32	0.15	0.12

N = Sample Size, Pred = Predicted product size (bp) from Barker [10], Size = Allele size range (bp), N_A = No. of Obtained Alleles, N_E = No. of Effective Alleles, I = Shannon's Information Index, H_O = Observed Heterozygosity, H_E = Expected Heterozygosity, HW = exact test of Hardy-Weinberg equilibrium with a significance at p = 0.01, F_IS = Inbreeding coefficient within individuals in each subpopulation, F_IT = Inbreeding coefficient of an individual relative to the total population, F_ST = Genetic differentiation among populations, G_ST = Analog of F_ST, D_est = Jost’s estimate of differentiation. ¹Mean over loci rather than the arithmetic average, ^NSnon significant, *P < 0.05; **P < 0.001 Probability values are based on 999 permutations.

ISSN: 1471-2229