Figure 5

The NSP2 and the miR171h promoter show distinct regulation in response to nutrients and mycorrhizal colonization. Roots were transformed with promoter-uidA fusions of either the MIR171h (A-D) or the NSP2 (E-I) promoter. After root transformation, chimeric plants were potted into substrate and grown under different nutritional regimes: phosphate starvation (B and F), phosphate starvation and mycorrhizal colonization (A and E), nitrogen starvation (C, G, and H) or full nutrition (D and I). A, A’ and A” as well as E, E’ and E’ represent identical root sections, with A’/E’ showing WGA Alexafluor 488 fluorescence to visualize R. irregularis structures and A”/E” showing overlay of bright filed images and WGA Alexafluor 488 fluorescence. Scale bars represent 100 μm (black and white) or 200 μm (blue). Arrowheads point to GUS staining in cortical cells containing arbuscules.