Schematic workflow of root hair isolation. Arabidopsis Col-0 plants were grown on cellophane disc for 4 or 5 days. The cellophane discs on which plants grew were transferred on the top of an aluminium tower placed in liquid nitrogen, left for 1-2 seconds, and plants except for root hairs were removed by brush. Root hairs attached on the cellophane disc were released in RNA extraction buffer. Other tissues such as root tips in the buffer were removed carefully with forceps under a stereomicroscope.