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Table 2 Time-course expression analysis a of Gmpgip genes in etiolated soybean hypocotyls infected with S . sclerotiorum

From: The pgip family in soybean and three other legume species: evidence for a birth-and-death model of evolution

Hours post infection (hpi)

Relative expressionb

Gmpgip3

Gmpgip5

Gmpgip7

8

1.16 ± 0.55

1.45 ± 1.95

4.38 ± 1.30

16

2.30 ± 0.891

0.56 ± 0.22

5.78 ± 0.17

24

5.76 ± 2.02

0.29 ± 0.32

6.98 ± 5.06

48

5.89 ± 2.69

454.0 ± 34.1

265.5 ± 32.01

  1. aAnalysis was performed by qRT-PCR. Gmpgip and housekeeping genes showed similar amplification efficiencies. Amplicon sizes: Gmpgip3 (211 bp); Gmpgip5 (194 bp); Gmpgip7 (200 bp); GmELF1A (195 bp).
  2. bQuantification of gene expression was performed using the comparative Ct method (Livak and Schmittgen, 2001). Relative expression of each gene is reported as the fold increase of the transcript level in infected sample relative to each corresponding non-infected control sample and normalized with GmELF1A.