Skip to main content
Figure 2 | BMC Plant Biology

Figure 2

From: Mass spectrometry analysis of the variants of histone H3 and H4 of soybean and their post-translational modifications

Figure 2

Determination of histone variant H3.1 and identification of methylation at Lysine 27 of histone variant H3.1. A. MALDI-TOF mass spectrum showing non- (m/z 915.52), mono- (m/z 929.53), di- (m/z 943.53) and tri- (m/z 957.55) methylation at Lysine 27 in the peptide 27KSAPATGGVK36 of histone H3.1. B, C, D and E. MS/MS spectrum of the peptide precursor ions at m/z 915.52, 929.53, 943.53 and 957.55 determining non-, mono-, di- and tri-methylation at Lysine 27 in the peptide of 27KSAPATGGVK36 of histone H3.1, respectively. These results clearly showed that the amino acid sequence of this peptide was KSAPATGGVK and only Lysine 27 was methylated, but not Lysine 36.

Back to article page