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Figure 4 | BMC Plant Biology

Figure 4

From: Interactions between cauliflower and Rhizoctonia anastomosis groups with different levels of aggressiveness

Figure 4

Safranin O and aniline blue staining of cauliflower hypocotyl cells after infection by seven different R. solani AGs and one binucleate Rhizoctonia AG. A, Cellular responses observed with safranin O and aniline blue staining were classified into three categories; photographs I-III depict representative examples. (I) Rhizoctonia hyphae are completely surrounded by cells fortified with safranin O positive material located in the cell walls or in granules observed in the cytoplasma, restricting further fungal growth. (II) Fortification of cell walls and presence of safranin O positive granules in the cytoplasma is observed for some adjacent cells, although colonization by Rhizoctonia hyphae is not stopped. (III) Absence of safranin O positive host responses in cells neighbouring Rhizoctonia hyphae. Scale bars = 50 μm. B, Frequency distribution of cellular response categories at 3, 6 and 12 dpi for different Rhizoctonia AGs. The three values within each cell represent the relative proportion of interaction sites designated as type I, II and III as detected after safranin O staining, respectively. C, Frequency distribution of cellular response categories at 3, 6 and 12 dpi for different Rhizoctonia AGs. The three values within each cell represent the relative proportion of interaction sites designated as type I, II and III as detected after aniline blue staining, respectively. At each time point, at least 50 interaction sites per AG were studied originating from 10 different cauliflower hypocotyls. Within one column, values followed by the same letter are not significantly different according to Kruskal-Wallis and Mann-Whitney tests (α = 0.05).

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