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Figure 1 | BMC Plant Biology

Figure 1

From: Identification of an extensive gene cluster among a family of PPOs in Trifolium pratense L. (red clover) using a large insert BAC library

Figure 1

T. pratense inserts released by digestion from 58 randomly selected BAC clones. Using Not1, DNA was separated by pulse-field gel electrophoresis (PFGE). BACs were generated by restricting T. pratense gDNA with HindIII, PFGE and cloning the size separated gDNA in the size region of 150–100 Kb. Molecular weight standards are lane 1, lambda ladder (NEB, Beverley, Mass., USA) and lane 2, DNA Molecular Weight Marker X (Roche); pIndigoBAC5 NotI vector fragment is 7 Kb. The average insert size calculated from all 11 BAC clones in lanes 3–13 is estimated as 113 Kb.

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