Figure 4
Examples of transposon ( mPing )-methylation display (TMD) profiles showing the tissue culture-induced alteration in cytosine methylation at the CCGG sites of the 5' mPing -flanking regions of the three rice ssp. indica cultivars. (a), (b) and (c) are profiles of cultivars V14, V27 and R09, respectively. Similar TMD profiles were obtained for the 3' mPing-flanking regions. The labeling of the four types of alteration in cytosine methylation at the immobile mPing-flanking regions are indicated as C1 – CG hypomethylation, C2 – CG hypermethylation, D1 – CHG hypomethylation, and D – CHG hypermethylation. The primer combinations are indicated at bottom of the profiles.