(a) PAGE showing purification of recombinant HaCS-fusion protein using Ni-NTA affinity chromatography. ni: uninduced control; ind: induced E. coli culture; ub: unbound fraction; w1-2: washing steps 1 to 2 using 20 mM imidazol; w3: washing step 3 using 100 mM imidazol; e1-3: elution steps 1 to 3 using 250 mM imidazol; M: marker. (b) GC-MS analysis (m/z 204) of an in vitro incubation of recombinant HaCS protein with FDP. Negative control: incubation of an E. coli protein extract not expressing HaCS under the same conditions. x: unidentified compounds.