Figure 6
Induction of NtMMP1 in wild type BY-2 suspension cells. BY-2 cells were treated with A. tumefaciens, P. syringae pv tomato DC3000 or xylanase from T. viridae. The bacteria were grown to OD600 of 1.0 and diluted 1:100 in the BY-2 cell culture. Xylanase was used at a final concentration of 2 μg/ml. Total RNA was extracted at the indicated time points and 12 μg were loaded per lane. NtMMP1 mRNA was detected by probing with a radiolabeled BglII/HindIII NtMMP1 fragment. Signals were detected with a phosphorimager and quantified using the AIDA software. The ethidium bromide bands confirm equal loading. Lane 1: 30 min untreated cells; lane 2: 30 min exposure to A. tumefaciens; lane 3: 30 min exposure to P. syringae; lane 4: 30 min exposure to xylanase; lane 5: 1 h untreated cells; lane 6: 1 h exposure to A. tumefaciens; lane 7: 1 h exposure to P. syringae; lane 8: 1 h exposure to xylanase.