Figure 3

GFP detection of hairy roots and regenerated transgenic plants. GFP-derived fluorescence detected by laser scanning confocal microscopy in a transgenic hairy root, scale bar = 70 μm (A) and in a negative control hairy root, scale bar = 300 μm (B). PCR-amplification of GFP in regenerated plants (C). M, 1 kb DNA marker; 1, plasmid DNA; 2, negative control; 3–7, transgenic regenerated plants. GFP-derived fluorescence detected by laser scanning confocal microscopy in a leaf from a regenerated plant, scale bar = 150 μm (D) and in a leaf from a negative control plant, scale bar = 150 μm (E). Western blot assay for the detection of GFP protein levels in independent transgenic plants using an anti-GFP antibody (F). 1, negative control plant; 2–8, independent transgenic plants. All negative controls were hairy roots or regenerated plants obtained by A. rhizogenes harbouring no binary vector mediated transformation.