Figure 1

Transgenic L. corniculatus cv. Superroot plants obtained from hairy roots induced by A. rhizogenes. Obtainment of transgenic L. corniculatus cv. Superroot by A. rhizogenes mediated transformation. Pre-cultivation of explants on MS medium (A). Co-cultivation of explants after infection with A. rhizogenes (B). Hairy roots began to appear at the wounding sites of the explants about 7 days after being transferred onto 1/2 MS medium. Pictures were taken 14 days after the first appearance of hairy roots (C). Plantlets/shoots regenerated from hairy roots on the RM 4 weeks later (D). Shoots were transferred onto MS medium for elongation (E). Shoot elongation and root formation about 4 weeks after being transferred onto MS medium (F). Comparison between transgenic L. corniculatus by A. rhizogenes-mediated transformation (left) and wild type plant (right) (G).