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Table 2 Number of T-DNA copies and 5'-tagged sequences in 'Three Hand Planty' lines transformed with promoter tagging vector pETKUL2

From: Characterization and isolation of a T-DNA tagged banana promoter active during in vitro culture and low temperature stress

  

No. 5'-tagged sequences

  

TAIL-PCR

I-PCR

Different sequencesa

ET2-Line

T-DNA copies

AD2

AD2-1

AD2-5

BsrGI

BclI

 

17

5

3

5

2

2

2

5

156

5

2

1

2

2

2

3

49

4

4

5

1

NT

4

6

179

4

2

3

3

3

5

4

85

3

2

1

1

2

NT

3

111

3

2

3

1

1

3

2

34

1

1

2

1

2

2

1

Average

3.3

2.3

2.9

1.6

2.0

3.0

3.4

  1. The minimum number of T-DNA copies was determined by Southern hybridization with a DIG-labelled luc+ probe. Isolation of 5'-tagged sequences was accomplished using three different arbitrary degenerated (AD) primers in TAIL-PCR and two different restriction enzymes in I-PCR.
  2. aNumber of different 5'-tagged sequences obtained.
  3. NT, not tested.