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Table 2 Number of T-DNA copies and 5'-tagged sequences in 'Three Hand Planty' lines transformed with promoter tagging vector pETKUL2

From: Characterization and isolation of a T-DNA tagged banana promoter active during in vitro culture and low temperature stress

   No. 5'-tagged sequences
   TAIL-PCR I-PCR Different sequencesa
ET2-Line T-DNA copies AD2 AD2-1 AD2-5 BsrGI BclI  
17 5 3 5 2 2 2 5
156 5 2 1 2 2 2 3
49 4 4 5 1 NT 4 6
179 4 2 3 3 3 5 4
85 3 2 1 1 2 NT 3
111 3 2 3 1 1 3 2
34 1 1 2 1 2 2 1
Average 3.3 2.3 2.9 1.6 2.0 3.0 3.4
  1. The minimum number of T-DNA copies was determined by Southern hybridization with a DIG-labelled luc+ probe. Isolation of 5'-tagged sequences was accomplished using three different arbitrary degenerated (AD) primers in TAIL-PCR and two different restriction enzymes in I-PCR.
  2. aNumber of different 5'-tagged sequences obtained.
  3. NT, not tested.