Figure 2

Improved thermotolerance and enhanced resistance to CDT in seeds from the DS10:A9/DR2 heterozygous lines. Segregation of hygromycin resistance (Hyg^R) was analyzed in germinating seeds before and after the indicated treatment. BTA, basal thermotolerance assays; CDT, controlled deterioration treatments. Experiments were performed at least in triplicate for each line. A. – Combined results from five lines with the DS10:A9#6-7 homozygous background (A9#6-7/DR2), four lines with the DS10:A9#14-5 background (A9#14-5/DR2), and eight DS10:DR2 lines (DR2) are shown. Segregation was analyzed five (in DS10:A9/DR2 lines) or eight days (in DS10:DR2 lines) after BTA. Segregation was analyzed five days after CDT. The expected segregation ratio for antibiotic resistance is indicated with a thick line (marking 3 Hyg^R: 1 Hyg^S). Asterisks indicate differences that were found to be statistically significant (P < 0.05). We show representative pictures of segregation of hygromycin resistance for the DS10:A9#6-7/DR2#22 line after BTA (B) and before BTA (C). Arrows in (B) indicate the few Hyg^S seedlings that survived BTA. Scale bars, 8 mm.