Fragments of the key flowering gene GIGANTEA are associated with helitron-type sequences in the Pooideae grass Lolium perenne

Table 1 PCR primer sequences, 5'....3'¹

GIG49660.6F	GTCCCGTCTATGATGCGTGA	GIG49660.7R	CCAGTTCTCATCACTGTTCTGG
GIGgt.1F	ATTCCTGCATCTGAAACCAC	GIGgt.1R	CAGCCAGCACATACGAGTC
GIGgt.2F	GCATCAAATGGGAAGTGGAT	GIGgt.2R	TGCAACTTTGAAGATTGGCC

¹Thermal cycling profile for all primer pairs was as follows: 1 minute at 94°C, followed by 10 cycles of 1 min at 94°C, 1 min at 60°C (with the temperature reduced by 1°C per cycle), 1 min at 72°C, followed by 30 cycles of 1 min at 94°C, 1 min at 50°C, 1 min at 72°C

ISSN: 1471-2229